The 3M™ Attest™ Ethylene Oxide Biological Indicator 1264, Green Cap, reliably monitors all ethylene oxide (EO, EtO) sterilization processes. It is compliant with ISO 11138-1:2017 and ISO 11138-2:2017 and offers a final result within 48 hours.
The ISO 11138-1:2017 and ISO 11138-2:2017 compliant 3M™ Attest™ Ethylene Oxide Biological Indicator, 1264, is a self-contained biological indicator (BI) that consists of a Bacillus atrophaeus spore strip and a sealed glass ampoule with growth media and bromothymol blue pH indicator dye system. The spore strip and media ampoule are contained in a plastic vial topped with a green color-coded cap which includes a chemical process indicator on the top.
When processed, the chemical process indicator turns from reddish-brown to green. After the ethylene oxide sterilization process, the BI is activated to join the growth media with the processed spore strip. The BI is then incubated in a 3M™ Attest™ Biological Indicator Incubator 127 for a visual color change readout at 48 hours. A color change from green to yellow indicates bacterial growth and a positive result. A final readout of a negative result (media remains green) is made after 48 hours of incubation.
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